How you can read in the title: How can I use and integrate a Python script in a backend from a web applicaton? The backend use php as programming language and the database MySql.
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Answer
If you want to run a python script from PHP, you can use one of the many PHP functions which do this, for example: exec().
Depending on what you want, it can be as simple as:
<?php
exec('python script.py');
?>